Research Paper Volume 15, Issue 7 pp 2373—2394
Characterization of the HDAC/PI3K inhibitor CUDC-907 as a novel senolytic
Figure 4. Mechanisms of cell death induced by CUDC-907 in senescent cells. (A, B) Representative Western blots of lysates of control (C) and senescent (S) EJp53 treated with different concentrations of CUDC-907 for 24h. Actin and H3 are used as a loading controls. (C) Cell viability of control and senescent EJp53 (top) and H522 (bottom) treated with different concentrations of rocilinostat for 72 hours. H522 were induced to senesce by exposure to 8 Gy of ionizing radiation and 6 days incubation. HCT116 were induced to senesce by exposure to 0.2 μM doxorubicin for 3 days. Values show mean ±SD of three independent experiments. (D) Representative Western blots of lysates of proliferating (C) and senescent (S) EJp53 treated with DMSO (control), 0.03 μM CUDC-907, 0.03 μM CI-994 (CI) or 0.03 μM panobinostat (Pano) for 24h. Actin and H3 are used as loading controls.