Research Paper Volume 15, Issue 7 pp 2373—2394

Figure 2. CUDC-907 has senolytic effects in different models of cellular senescence. (A) Cell viability of control (proliferating, blue) and senescent (6 days after tet removal, red) EJp53, EJp21 and EJp16 cells after treatment with different concentrations of CUDC-907 for 72h, as measured by a CTG assay. (B) Induction of apoptosis by different concentrations of CUDC-907 in control or senescent EJp53, as measured by Annexin V staining and FACs analysis. The percentages of Annexin V-positive cells are plotted. (C) Cell viability of control and senescent EJp53 after CUDC-907 treatment in the presence of DMSO (Control) or 10 μM of QVD-OPH (CI), as measured by a CTG assay. (D) Cell viability of control and senescent H522 and HCT116 72h after treatment with CUDC-907, as measured by a CTG assay. H522 were induced to senesce by exposure to 8 Gy of ionizing radiation and 6 days incubation. HCT116 were induced to senesce by exposure to 0.2 μM doxorubicin for 3 days. All values in this figure show mean ±SD of three independent experiments, and P values between each control and senescence pair are shown as *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.