Research Paper Volume 15, Issue 6 pp 2208—2220

Figure 2. GALNT2 is a downstream gene of STAT3. (A, B) Both GALNT2 mRNA (A) and protein (B) levels were upregulated in GSCs as compared with their corresponding non-GSC cells. (C, D) Silencing STAT3 dramatically inhibits GALNT2 mRNA (C) and protein (D) expression. sh NC means non targeting control shRNA, sh STAT3 indicates STAT3 shRNA. (D, E) GALNT2 mRNA (E) and protein (D) expression was inhibited by treated GSC1 cells with 5 mM GSK343 for 48 h. (F) Luciferase construct containing full-length of GALNT2 promoter and STAT3 overexpression plasmid was co-transfected into GSC1 cells for 48 h. Transcriptional activation of STAT3 was determined by dual-luciferase reporter assay. (G) STAT3 directly binds to the GALNT2 promoter. (H) GALNT2 mRNA was positively correlated with STAT3 mRNA in the TCGA database. MW indicates molecular weight. *** p < 0.001.