Research Paper Volume 15, Issue 6 pp 2136—2157

Figure 2. Prognostic roles of CDKN2A in pan-cancer. (A) Summary of the correlations between CDKN2A expression and overall survival (OS), disease-free interval (DFI), disease-specific survival (DSS) and progression-free interval (PFI) of cancer patients according to univariate Cox regression and Kaplan-Meier model. Red indicates that CDKN2A is the risk factor affecting prognosis of cancer, and green indicates a protective factor. Only p value < 0.05 are colored. (B) Through univariate Cox regression method, forest map shows the prognostic role of CDKN2A in cancer. The red cancer type indicates that CDKN2A is a statistically significant risk factor. (C–F) Kaplan-Meier total survival curve of CDKN2A in ACC-OS (C), HNSC-OS (D), KICH-OS (E) and LGG-OS (F). (G) Immunohistochemical images showed the expression and location of p16 protein in six pairs of LGG and adjacent tissues. (H) Paired-t test showed the statistical significance of p16 positive rate of the IHC results. (I) Western blot validated the knock-down effects of the siRNAs in SW1088 cells. (J, K) CCK8 cell proliferation analysis (J) and cell colony formation assay (K) showed the discrepancy of cell proliferation abilities between SW1088 cells with or without CDKN2A knock-down.