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Research Paper Volume 15, Issue 6 pp 2097—2114
ADAMTS1 induces epithelial-mesenchymal transition pathway in non-small cell lung cancer by regulating TGF-β
Figure 6. ADAMTS1 positively regulates the expression of TGF-β in NSCLC cells or tumor tissues. (A) Immunofluorescence was used for detecting ADAMTS16 and TGF-β in NSCLC tissues from different grades. Scale bar=200 μm. (B) qRT-PCR showed the mRNA expression level of TGF-β in lung tissues and adjacent normal tissues. (C) qRT-PCR showed the mRNA expression level of TGF-β in A549 cells overexpressing ADAMTS1. (D) Secretion of TGF-β protein in A549 cells detected using ELISA. (E) Protein expressions of TGF-β, LAP-TGF-β, and p-Smad2/3 in A549 cells were detected using Western blot. (F) qRT-PCR showed the mRNA expression level of TGF-β in H226 cells overexpressing ADAMTS1. (G) Secretion of TGF-β protein in H226 cells detected using ELISA. (H) Protein expressions of TGF-β, LAP-TGF-β, and p-Smad2/3in H226 cells were detected using Western blot. **P<0.01, ***P<0.001 compared with NC group. N=3.