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Research Paper Volume 15, Issue 6 pp 2097—2114
ADAMTS1 induces epithelial-mesenchymal transition pathway in non-small cell lung cancer by regulating TGF-β
Figure 4. ADAMTS1 promotes migration, invasion, and EMT of BEAS2B cells. ADAMTS1 overexpression plasmids or si-ADAMTS1 was transfected into BEAS2B cells. (A) Wound-healing assay was used for detecting the migration of BEAS2B cells transfected with ADAMTS1 overexpression plasmids. (B) Transwell examined the migration and invasion of BEAS2B cells (scale bar=50 μm). (C) Protein expressions of E-cadherin, N-cadherin, and Vimentin in BEAS2B cells were detected using Western blot. NC=negative control. (D) The wound-healing assay showed the migration of BEAS2B cells after the knockdown of ADATMS1. (E) Transwell assays showed the migration and invasion of BEAS2B cells after the knockdown of ADATMS1 (scale bar=50 μm). (F) Protein expressions of E-cadherin, N-cadherin, and Vimentin in BEAS2B cells were detected using Western blot. (G) EdU staining was used for detecting cell proliferation. Scale bar=100 μm. *P<0.05, **P<0.01, ***P<0.001 compared with NC or si-NC group. N=3.