Research Paper Volume 15, Issue 6 pp 2033—2045

Figure 5. Epimedin C lost its function without Nrf1 expression. (A) OB cells probed by RhoA antibody, WT and CRISPR-Nrf1 treated with Epimedin C and DXMS in different dosage. (B) The Luc signal captured in OB cells. WT and CRISPRE-Nrf1 group treated with Epimedin C and DXMS, the Luc ORF drive by RhoA promoter exhibits the positive signal in different level. (C) Compared with WT group, the CRISPR-Nrf1 OB cells got a lower expression level of RhoA protein, even in the Epimedin C treatment condition.