Research Paper Volume 15, Issue 5 pp 1668—1684

Figure 6. NMU promotes PTC cell viability, colony formation, cell proliferation abilities and tumor growth. (A) Suppression of NMU by NMU-siRNAs (siNMU#1 and siNMU#2) determined by RT-qPCR. (B) NMU-siRNAs significantly reduced cell viability of TPC-1 and KTC-1 cells compared to control group. (C) NMU-siRNAs significantly reduced colony formation number in TPC-1 and KTC-1 cells. (D) The inhibitory effect of NMU-siRNAs on cell proliferation was confirmed with the EdU assay (^*p < 0.05; ^**p < 0.01; ^***p < 0.001). (E) Subcutaneous xenograft tumors formed by TPC-1 cells in nude mice. siRNA transfection was adopted to reduce NMU levels in tumors. Tumor weights and tumor diameter were measured for each group (n = 4 per group). NMU-siRNAs significantly inhibited the growth of tumors in nude mice. (^**p < 0.01; ^***p < 0.001).