Research Paper Volume 15, Issue 5 pp 1652—1667

Figure 5. ANGPTL2 increases VEGF-A synthesis in lung cancer cells via the integrin α5β1 receptor, p38 and NF-κB signaling. (A–C) Cells were transfected with ANGPTL2 cDNA, then stimulated with LILRB2 and integrin α5β1 siRNA or SB203580 and PDTC; VEGF-A expression was measured by Western blot (n=3). Quantitative data of the protein level were obtained using ImageJ software. Densitometric analysis of protein expression was normalized to β-actin. (D, E) Cells were transfected with ANGPTL2 cDNA, then stimulated with integrin α5β1 siRNA and SB203580; p38 and NF-κB activation was measured by Western blot (n=3) and NF-κB luciferase activity. Quantitative data of the protein level were obtained using ImageJ software. Densitometric analysis of protein expression was normalized to β-actin. *p < 0.05 compared with CL1-0; #p < 0.05 compared with Control.