Research Paper Volume 15, Issue 8 pp 2920—2936
ESM1 promotes angiogenesis in colorectal cancer by activating PI3K/Akt/mTOR pathway, thus accelerating tumor progression
Figure 5. Endothelial angiogenesis ability under SW480 and SW620 stimulation, and detection of effect of ESM1 on the proliferation of SW480 and SW620 cells via flow cytometry. (A) Number of endothelial cell tube formation in 3B-11 cells co-cultured with SW480 cells transfected with ESM1-NC and ESM1-mimic. (B) Number of endothelial cell tube formation in 3B-11 cells co-cultured with SW620 cells transfected with ESM1-NC and ESM1-mimic. (C, D) Flow cytometry reveals that ESM1-mimic can significantly increase the ratio of G2 phase cells and promote cell proliferation. *p < 0.05 and **p < 0.01 vs. ESM1-NC group.