Research Paper Volume 15, Issue 4 pp 1052—1073

Figure 5. TIPE2 suppresses the progression of EOC via inhibition of the PI3K/Akt/GSK3β signaling pathways. (A) 49 upregulated and 28 downregulated genes in the SKOV3/TIPE2 group compared with the vector group on |Foldchange| > 0 and adjusted p < 0.05. (B) KEGG pathway annotation of differentially expressed genes. (C, D) Western blot analysis of PTEN, p-PI3K, PI3K, p-AKT, AKT, p-GSK3β, GSK3β and p53. The whole lysates derived from TIPE2 and vector infected HeyA8 and SKOV3 human EOC cells were immunoblotted with a panel of antibodies specific for PTEN, p-PI3K (Tyr458), PI3K, p-Akt (Ser473), Akt, p-GSK3β (Ser9), GSK3β, p53 and β-actin (a loading control) (n=3). Data are presented as mean ± SD. Statistical significance is indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.