Research Paper Volume 15, Issue 4 pp 1052—1073

Figure 3. Overexpression of TIPE2 suppresses ovarian cancer cell growth and increased cell apoptosis. (A) Imagines of colony formation. 1×10³ HeyA8 and SKOV3 cells transfected with TIPE2, and its control plasmid mock were cultured to a new well with medium containing 10% FBS serum, and cell colony formation was examined by staining with crystal violet (n=3). (B) Cell viability of HeyA8 and SKOV3 cells with stable expression of TIPE2 or vector control was also determined by CCK8 assay (n=3). (C) Cell apoptosis was detected using flow cytometry. (Annexin V+/7-AAD-, early apoptosis; Annexin V+/7-AAD+, late apoptosis). Data are presented as mean ± SD. Statistical significance is indicated as: *p < 0.05, **p < 0.01, ****p <0.0001.