Research Paper Volume 15, Issue 4 pp 1052—1073

Figure 2. Overexpression of TIPE2 in EOC cells by lentivirus transfection. (A) Stable transfected EOC cell lines were established by lentivirus transfection. Representative images of stably transfected cells in bright field and dark fields are shown. Fluorescence microscopy showed that transfection efficiency was approximately 80%, and the cell state was normal. (B) qRT-PCR was conducted to compare endogenous TIPE2 expression between EOC cells transduced with lentivirus (n=3). TIPE2 expression was normalized to β-actin control. (C) TIPE2 protein expression in HeyA8 and SKOV3 cells by western blot analysis. β-actin was used as an internal control. Data are presented as mean ± SD. Statistical significance is indicated as: *p < 0.05, ***p < 0.001.