Research Paper Volume 15, Issue 5 pp 1358—1370
Ginkgolide A improves the pleiotropic function and reinforces the neuroprotective effects by mesenchymal stem cell-derived exosomes in 6-OHDA-induced cell model of Parkinson’s disease
Figure 6. GA pre-treated WJMSCs exosomes repair excessive autophagy in 6-OHDA stimulated model. (A) The intensity and puncta of LC3-B were measured by immunofluorescence and DAPI staining. The white arrows indicate the dots showing increased puncta. (B) Immunoblot assays were performed to examine the Beclin-1, p62, and LC3-B proteins in the 6-OHDA damage model. (C) Immunofluorescence staining with alpha-synuclein p-129 anti-body after alpha-synuclein recombinant protein treatment in 6-OHDA damage model in SH-SY5Y cells. The white arrows indicate the expression of alpha-synuclein p-129. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control and #P < 0.05, ##P < 0.01, ###P < 0.001 vs. 6-OHDA groups.