Research Paper Volume 15, Issue 6 pp 1713—1733
Knockout of AMD-associated gene POLDIP2 reduces mitochondrial superoxide in human retinal pigment epithelial cells
Figure 4. Knockout of POLDIP2 did not significantly alter autophagy in ARPE-19. (A) Western blot analysis of LC3B protein levels in WT and POLDIP2 KO samples. E64d and pepstatin A protease inhibitors (10 mg/ml each) were added where indicated. β-actin served as a loading control. (B) Quantification of the ratio of LC3B-II to β-actin. Values expressed as mean ± SEM, n=3 biological repeats. n.s. not significant. (C) Quantification of the ratio of LC3B-II/(LC3B-I + LC3B+II). Values expressed as mean ± SEM, n=3 biological repeats. n.s. not significant.