Research Paper Volume 15, Issue 3 pp 650—674

Figure 1. Loss of hyl-2/CER synthase results in poor oxidative stress responses. (A) Diagram showing the lipid pathways and metabolic enzymes producing free fatty acids (FFAs) and sphingolipids (SLs). (B) Survival of 1-, 5-, and 10-day old N2 (blue), asm-3 (red), and hyl-2 (green) worms treated with 150μM juglone. Worms were treated with juglone in 96 well plates, and survival was determined by a movement response to agitation. Survival curves were analyzed using Kaplan-Meier estimate and pairwise differences were determined using log-rank tests (n=49 for N2 1 day; n=48 for N2 10 day; n=46 for asm-3 1 day; n=35 for asm-3 10 day; n=28 for hyl-2 1 day; n=44 for hyl-2 10 day). (C) Representative images (left) and fluorescence quantification (right) of Nile Red lipid staining in 1-day and 10-day old N2, asm-3, and hyl-2 animals. ^#indicates p<0.05 compared to 1-day counterpart of the same genotype and *indicates p<0.05 compared to N2 counterpart of the same age.