Research Paper Volume 15, Issue 3 pp 718—733
Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts
Figure 5. Effect of IL-6 and/or rapamycin on BMP9-induced bone formation and bone defects repair. (A) Effect of BMP9 and/or IL-6 on growth plate was measured using fetal limb culture (PZ: proliferation zone, HZ: hypertrophic zone; Scale bar: 250 μm). mTOR phosphorylation (p-mTOR) was measured using immunofluorescent stain and confocal assay. (B) Effect of BMP9, IL-6, and/or rapamycin on bone defects repair was measured using μ-CT scan. (C) Quantification analysis μ-CT scan shows the effect of BMP9, IL-6, and/or rapamycin on bone defects repair. (D) Effect of BMP9, IL-6, and/or rapamycin on bone defects repair was measured using Masson trichrome stain (Scale bar: 250 μm). (Rapamycin: mTOR inhibitor. BMD: Bone mineral density, BS/TV: Bone surface density, BV/TV: Percent bone volume, Tb.N: Trabecular number, Tb.Sp: Trabecular separation). (* P < 0.05).