Research Paper Volume 15, Issue 2 pp 492—512

Figure 4. M3541 is a strong enhancer of inflammatory signaling in irradiated cancer cells. (A) Relative gene expression in A549 cells treated with DMSO, M3541, IR or IR+M3541 for 7 days measured by quantitative PCR. Data are shown as mean±SEM. (B) Western blot analysis of cGAS/STING pathway in A549 cells after DMSO, M3541, IR or IR+M3541 treatment for 7 days. (C) Relative gene expression in A549 cells treated as above with or without 2 μM TBK1 inhibitor, MRT67307 for 7 days. Data are shown as mean±SEM. (D) Relative expression of PD-L1 in A549, A375, HeLa and H1299 cancer cells treated as in (A). (E) Changes in cytokine levels in the culture media from A549 cells exposed to DMSO, M3541, IR or IR+M3541 for 7 days. Cytokine levels were measured by the MSD technology, normalized to the total cell number and presented as pg/ml per million cells. (F) Heatmap of the relative gene expression in 6 cancer cell lines exposed to M3541, IR or IR+M3541 for 7 days.