Research Paper Volume 15, Issue 2 pp 542—552

Figure 2. Metformin increased MAT in C57BL/6 and ob/ob mice. Adipocyte size was assessed in high-power field images of the proximal tibia. Representative images of HE staining (A–D) and oil red O staining (E–H) for each group. (I) Area of adipocytes represented as mean ± SEM. (J) The number of adipocytes per square millimeter. mRNA relative expression of adipogenic genes (Ppar-γ, Cebp-α, Scd1) (K) and osteogenic genes (RunX2, OPN, OCN) (L) in the bone marrow. Data are presented as mean ± S.E.M. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. c57 ctr. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. ob ctr.