Research Paper Volume 15, Issue 2 pp 441—458
Clearance of p16Ink4a-positive cells in a mouse transgenic model does not change β-cell mass and has limited effects on their proliferative capacity
Figure 4. Senolysis restored proliferative capacity in a subset of animals after a HFD metabolic challenge in 1-year old mice. (A) Correlation between percentage of non-senescent FLAG− islets and beta-cell proliferation in 12-month-old animals. Line of best fit is shown along with dotted lines indicating 95% confidence intervals. P-value was calculated using the null hypothesis that the slope of the best-fit line equals 0. (B) Pancreatic weight of the groups; (C) beta-cell mass calculated in (mg). (D) Beta-cell proliferation calculated by %BrDU+ Ins+ cells. Mean+/− SEM; significance calculated by ordinary one-way ANOVA with Tukey’s multiple comparisons. (E) Comparison between responders and non-responders to BB/homodimerizer treatment in the HFD group. AUC for IPGTT before treatment. (F) Blood glucose percentage changes compared to the beginning of the treatment and AUC (G) between responders and non-responders.