Research Paper Volume 15, Issue 1 pp 246—260
Mitoquinone shifts energy metabolism to reduce ROS-induced oxeiptosis in female granulosa cells and mouse oocytes
Figure 3. Effect of MitoQ on ROS-induced oxeiptosis. (A) Flow cytometry was performed on MitoQ treated for 24 h, followed by 0.8 mM H2O2 treatment for 4 h and double staining by annexin V/PI. (B) Quantification of annexin V/PI double-stained cell populations in each quadrant, respectively. (C) Analysis of protein levels in granule cells by immunoblotting. (D) The time-lapse images are used to capture pictures of cell morphology at different times. CL: cleavage form. Scale bar: 25 μm.