Research Paper Volume 15, Issue 1 pp 179—192
lncRNA HCG11 suppresses cell proliferation in hormone receptor-positive breast cancer via SRSF1/β-catenin
Figure 5. SRSF1 promoted Wnt signaling via upregulating β-catenin expression. (A) Anti-SRSF1 RIP assay was applied to validate the binding of SRSF1 to β-catenin mRNA. (B) Anti-Flag RIP assay was applied to validate the binding of SRSF1 to β-catenin mRNA. (C, D) mRNA level of β-catenin in MCF-7 and BT-474 cell line transfected with SI-SRSF1. (E–H) Protein level of β-catenin in MCF-7 and BT-474 cell line transfected with SI-SRSF1. (I–K) Protein level of c-Myc and cyclin D1 in MCF-7 and BT-474 cell line transfected with SI-SRSF1. *p<0.05, **p<0.01, ***p<0.001, **** p<0.0001, ns represents p>0.05.