Figure 2. HCG11 inhibited the proliferation of HR-positive BC cells in vitro. (A) Expression of HCG11 was confirmed by qRT-PCR in MCF-7 and BT-474 cell line transfected with SI-NC or SI-HCG11. (B, C) Effect of SI-HCG11 on proliferation in MCF-7 and BT-474 cell line by CCK8 assay. (D, E) Effect of SI-HCG11 on proliferation in MCF-7 and BT-474 cell line by colony formation assay. (F, G) Effect of SI-HCG11 on proliferation in MCF-7 and BT-474 cell line by western blot. *p<0.05, **p<0.01, ***p<0.001, **** p<0.0001, ns represents p>0.05.