Research Paper Volume 15, Issue 1 pp 164—178

Figure 5. Dox-induced mitochondrial ROS stabilizes HIF1A through the downregulation of PHD and promotes IGFBP3-induced cardiac apoptosis. (A) H9c2 cells and tissues from rats administered with indicated concentrations of Dox were subjected to western blotting. (B, C) Dox-exposed cells were treated with the ROS scavenger NAC (B), mitochondria complex I inhibitor rotenone (Rot) or the NADPH oxidase inhibitor Apo (C). The harvested cellular extract was analyzed using western blotting. (D) Cells were transfected with a PHD overexpression plasmid of the indicated amount and levels of HIF1α, IGFBP3, pro-survival, and pro-apoptotic proteins in cells and cell culture medium (for secreted IGFBP3) were measured by western blot analysis. Data represents as the mean ± standard deviation of the mean (n = 3). Statistical significance is represented as follows: ^*P < 0.05, ^**P < 0.01.