Research Paper Volume 14, Issue 24 pp 9877—9889
SOX2-high cancer cells exhibit an aggressive phenotype, with increases in stemness, proliferation and invasion, as well as higher metabolic activity and ATP production
Figure 4. Glycolysis is significantly enhanced in SOX2-high MCF7 cells. MCF7 cells stably-transduced with the SOX2-GFP construct were subjected to FACS sorting to isolate the 5% highest GFP (GFP-high) and the 5% lowest GFP (GFP-low) subpopulations. The Seahorse XF96 analyser was employed to measure glycolytic function. In the top panel, a representative ECAR tracing is shown. In the lower panel, bar graphs show the glycolytic rate, glycolytic capacity and glycolytic reserve, obtained from the ECAR quantification. Experiments were performed at least 3 times independently. Results are shown as the mean ± SD: t-test, two tailed-test, *p < 0.05, ** p < 0.01.