Research Paper Volume 14, Issue 24 pp 9860—9876

Figure 5. C-C motif chemokine ligand 2 (CCL2) restores myotubes (MTs) damaged by dexamethasone. (A) Quantitative reverse-transcription polymerase chain reaction analyses (qRT-PCR) of myogenin and myosin heavy chain (MyHC) in C2C12 cells treated with 2% horse serum, with and without MTs damaged by dexamethasone or the indicated concentrations of recombinant CCL2 for 2 days. (B) MTs were stained with anti-MyHC antibody, and the nuclei were counterstained with 4,6-diamidino-2-phenyindole (DAPI). Scale bars, 100 μm. (C) Quantitative results per field are presented. Number of experiments (No.) of (A) = 4 times. No. of (B, C) = 6 times, respectively. Data are expressed as mean ± standard deviation (SD). (A, C) *P<0.05, **P<0.005, ***P<0.0005, †P<0.01, ††P<0.001, †††P<0.0001 vs. CCL2 '–' & Dexa ‘– ‘(vehicle: PBS + 0.1% BSA), or vs. CCL2 '–' & Dexa 200μM (represented with lines).