Research Paper Volume 14, Issue 24 pp 9860—9876

Figure 2. Recombinant human C-C motif chemokine ligand 2 (hCCL2) promotes cell viability, proliferation, and myogenesis in human skeletal muscle myoblast (HSMM) primary human cells. (A) HSMM cell viability on Cell Counting Kit-8 (CCK-8) assay and proliferation on 5-bromo-2′-deoxy-uridine (BrdU) assay. (B) Quantitative reverse-transcription polymerase chain reaction analyses (qRT-PCR) of myogenin and myosin heavy chain (MyHC) as a marker for myotube (MT) formation. (C) MTs were stained with an anti-MyHC antibody, and the nuclei were counterstained with 4, 6-diamidino-2-phenyindole (DAPI). Scale bars, 100 μm. (D) Quantitative results per field are presented. The number of experiments (No.) of (A, B) = 4 times, respectively. No. of (C, D) = 6 times, respectively. Data are expressed as mean ± standard deviation (SD). (A, B) *P<0.05, **P<0.005, ***P<0.0005, †P<0.01, ††P<0.001, †††P<0.0001 vs. hCCL2 '0' (Vehicle: PBS + 0.1% BSA). (C, D) *P<0.05, **P<0.005, ***P<0.0005, †P<0.01, ††P<0.001, †††P<0.0001 vs. Day 5 of hCCL2 '0' (vehicle: PBS + 0.1% BSA).