Research Paper Volume 14, Issue 24 pp 9832—9859

Figure 1. Transcriptomic profiling and differential expression of linear and circRNA transcripts in ALS muscle. (A) Schematic of the RNA-seq workflow (blue boxes) and validation of candidate circRNAs (green boxes). (B) PCA performed on linear coding and long noncoding transcripts of human muscle biopsies from 5 normal and 5 ALS individuals. The age, sex, and muscle biopsy site are also listed (Abbreviations: y.o.: year old; F: female; M: male; BI: biceps brachii; DL: deltoid). (C, D) Differential expression of linear coding and noncoding transcripts (C) or circRNAs (D) in the ALS cohort by RNA-seq analysis. For (C), significance was established based on adjusted p-value < 0.05, whereas for (D) significance was established based on an unadjusted p-value < 0.05.