Research Paper Volume 14, Issue 24 pp 10027—10049

Figure 6. Hypomethylation status correlated with upregulation of MCM8 expression and poor survival in HCC patients. (A, B) DNA methylation level of MCM8 gradually decreased with the progression of tumor stages (A) and histologic grades (B). (C) The MCM8 methylation level is lower in metastatic lymph nodes than without metastasis. (D) The MCM8 methylation level is lower in HCC with TP53 mutation. (E) The heat map shows CCT7-related methylated CpG sites in HCC. (F) Methylation level of three CpG sites were associated with overall survival times of HCC patients. (G–I) MCM8 mRNA expression negatively correlated with methylation levels of cg03098629 (G), cg10518808 (H) and cg17230679 (I). (J–L) Hypermethylation of cg03098629 (J), cg10518808 (K) and cg17230679 (L) was associated with better overall survival times.