Research Paper Volume 14, Issue 23 pp 9393—9422
Single nuclei profiling identifies cell specific markers of skeletal muscle aging, frailty, and senescence
Figure 2. Single-nuclei sequencing reveals 7 clusters of unique cell types, and differential gene expression with age. (A) Uniform Manifold Approximation and Projection (UMAP) of 5′ single nuclei sequencing of human muscle. All samples are shown, after data normalization and Louvain clustering. (B) Top 20 differentially expressed genes (DEG), in old vs. young samples. All cells from all cell types are used in this test. Wilcoxon test, top 20 DEGs by logFC. (C) Expression of PDK4 and IGFNl in young and old samples.