Figure 1. D-gal and OSS-128167 increased ROS production and HK2 and β-gal expression and decreased TERT expression in H9c2 cells, whereas C3G and UBCS039 increased TERT expression and decreased ROS levels and HK2 and β-gal expression. HK2 (A) and TERT mRNA levels in H9c2 cells (B) were detected using real-time PCR, ROS levels (C) were detected with a ROS assay, and β-gal expression (D) was observed using cytochemistry followed by semiquantitative analysis (E). Magnification, 10×. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.