Figure 4. Effects of exercise on arrhythmias and lifespan. (A–C) Heart rate, heart period, and arrhythmia indices in exercise-treated 14- and 35-days-old flies in the W1118. N = 30. (D–F) Heart rate, heart period, and arrhythmia index in exercise-treated 14- and 35-days-old flies in the LSP2-Gal4. N = 30. (G–I) Heart rate, heart period, and arrhythmia index in exercise-treated flies in the LSP2-Gal4>W1118. N = 30. (J) The lifespan of exercise-treated 14- and 35-days-old flies in the W1118. On the left is the fly′s survival rate (%), and on the right is the average lifespan of flies. The sample sizes for Control and exercise were 202 and 210. (K) The lifespan of exercise-treated flies in the LSP2-Gal4. On the left is the fly′s survival rate (%), and on the right is the average lifespan of flies. The sample sizes for Control and exercise are 190 and 193. (L) The lifespan of exercise-treated flies in the LSP2-Gal4>W1118. On the left is the fly′s survival rate (%), and on the right is the average lifespan of flies. The sample sizes for Control and exercise were 205 and 181. P-values for all survival curves were obtained from the log-rank test. All P-values except survival curves were from student t-tests, and all values are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.