Research Paper Volume 14, Issue 24 pp 9908—9923

Figure 1. Older flies are prone to heart rate disorders and disorders of lipid metabolism. (A–C) Heart rate, cardiac cycle, and arrhythmia index of 14- and 35-days-old flies in the background of W¹¹¹⁸. N = 30. The between-group comparisons were from student t-tests. (D) M-mode traces (8 s) prepared from high-speed movies of intact flies. The yellow arrows mark the SI, DI, HP, namely the systolic interval, the diastolic interval, and the heart period, respectively. This notation applies to all M-mode traces in this study unless otherwise stated. (E) Climbing index of 14- and 35-days-old flies. N = 50, see the “Materials and methods” section to calculate the climbing index. (F) Whole-body TG levels in 14- and 35-days-old flies. N = 5. Error bars represent three independent replicates. (G) Body weight of 14- and 35-days-old flies. N = 5, values are expressed as the body weight of 5 flies and measured in triplicate. (H) Whole-body apoLpp mRNA expression levels in 14- and 35-days-old flies. GAPDH was used to normalize these values, N = 10. Between-group comparisons were from student t-tests. All values except body weight are expressed as mean ± SEM, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.