Research Paper Volume 14, Issue 23 pp 9679—9698

Figure 7. SAFB is required for SKA1-mediated aggressive phenotype of ccRCC. (A) Transwell assay was used to assess the invasiveness and migration of SKA1 overexpressing cells co-transfected siSAFB or scrambled control plasmid. (B) Transwell assay was used to assess the invasiveness and migration of SKA-depleted cells co-transfected SAFB overexpression or control plasmid. (C) Western blot analysis of E-cadherin and vimentin protein levels in cells transfected with indicated constructs after TGF-β1 treatment. One representative experiment of three independent experiments are shown. (D) Luciferase activity was determined in SKA-depleted cells co-transfected SAFB overexpression or control plasmid. (E) Luciferase activity was determined in SKA overexpressing cells co-transfected siSAFB or control plasmid. Data shown are mean from three independent experiments performed in triplicate. ^*P < 0.05.