Research Paper Volume 14, Issue 23 pp 9679—9698

Figure 2. Knockdown of SKA1 attenuates tumor cell motility in vitro and in vivo. (A and B), Effects of SKA1 on the invasion and metastasis of RCC cells were confirmed by Transwell assay and wound healing assay. (C) The subcutaneous xenotransplantation model was established with 786-O cell line transfected with sh-SKA1 or sh-Ctrl. The length (L) and width (W) of tumors were measured once every four days for seven times after a six-week inoculation. At the end of investigation, mice were sacrificed and xenografts were then collected for diameter and weight measurements. (D) SKA1 and vimentin staining were performed in xenograft, scare bar: 100 μm. (E) Quantitation of tumor burdens determined by bioluminescence intensity, and numbers of metastatic nodules were counted in each group.