Research Paper Volume 15, Issue 8 pp 2877—2890
LINC01296 promotes proliferation of cutaneous malignant melanoma by regulating miR-324-3p/MAPK1 axis
Figure 7. LINC01296 enhanced the proliferation abilities of CMM cells by regulating MAPK1. (A) qRT-PCR was conducted to verify the relative expression of MAPK1 in M21 and A2058 cells transfected with sh-NC+pcDNA-NC, sh-NC+pcDNA-MAPK1, sh-LINC01296+pcDNA-MAPK1. (B) The expression of MAPK1 was analyzed by Western blotting with the indicated antibodies and samples from the M21 and A2058 cells in different transfected groups. (C) CCK-8 assay of M21 and A2058 cells in different transfected groups. (D) Cell proliferation was determined by colony-formation assay of different transfected groups in M21 and A2058 cells. All of data were analyzed from three independent experiments. * P < 0.05; ** P < 0.01.