Research Paper Volume 14, Issue 22 pp 8944—8969
JAK2/STAT3 pathway mediates neuroprotective and pro-angiogenic treatment effects of adult human neural stem cells in middle cerebral artery occlusion stroke animal models
Figure 7. STAT3 signaling pathway activated by ahNSCs in vivo and in vitro. (A) Expression of p-STAT3 in the infarct lesion of MCAO animal models (n = 3 per group) was determined by western blotting analysis at 28 d after ahNSCs transplantation. Left. Representative images of western blot analysis are shown. β-actin (43 kDa) = loading control. Right. Expression of p-STAT3 (79/86 kDa) was normalized by STAT3 and then compared. Mean ± SD. ** P < 0.01, *** P < 0.001. (B) Primary cortical neurons (n = 3 per group) were cultured in OGD condition for 1 h and then incubated in IM or NSC-CM for 24 h. Expression of p-STAT3 of primary cortical neurons was determined by western blotting analysis. Left. Representative images of western blot analysis are shown. β-actin (43 kDa) = loading control. Right. Expression of p-STAT3 (79/86 kDa) was normalized by STAT3 and then compared. Mean ± SD. *** P < 0.001.