Research Paper Volume 14, Issue 22 pp 8944—8969

Figure 3. Effects of ahNSCs on density of neurons, astrogliosis, and microglial activation. (A) Ipsilateral infarct and contralateral non-infarct areas examined for each animal are illustrated. (B) The density of neurons was examined by immunofluorescence against NeuN at 28 d after ahNSCs transplantation. Representative images illustrate each group (n = 5 for the control group, n = 6 for the HBSS group, n = 5 for the NSC group). Scale bar = 100 μm. (C) The numbers of NeuN-positive cells were calculated and then compared. Mean ± SEM. ** P < 0.01. (D) Astrogliosis was examined by immunofluorescence against GFAP at 28 d after ahNSCs transplantation. Representative images illustrate each group (n = 5 for the control group, n = 6 for the HBSS group, n = 5 for the NSC group). Scale bar = 100 μm. (E) The numbers of GFAP-positive cells were calculated and then compared. Mean ± SEM. * P <0.05; *** P < 0.001. (F) Microglial activation was examined by immunofluorescence against Iba1 at 28 d after ahNSCs transplantation. Representative images illustrate each group (n = 5 for the control group, n = 6 for the HBSS group, n = 5 for the NSC group). Scale bar = 100 μm. (G) The numbers of Iba1-positive cells were calculated and then compared. Mean ± SEM. * P <0.05.