Research Paper

Figure 7. Adipocyte-derived exosomal miR-30c-5p exacerbates endocrine and metabolic disorders in mice with PCOS. (A) Procedure of animal experiments. (B) Visualization of uptake of exosomes by the ovaries in vivo. (C) qRT-PCR detection of miR-30c-5p levels in the ovaries of mice in the indicated groups. (D) Estrous cycle analyses of mice in each group via vaginal smears. (E) Weight change in the indicated groups after administration of exosomes and miR-30c-5p antagomir for 1 and 2 weeks. (F) Fasting insulin levels in the indicated groups. (G) Fasting glucose levels in the indicated groups. (H) HOMA-IR index in the indicated groups. HOMA-IR = fasting insulin × fasting glucose/22.5. (I–L) ELISA detection of serum E₂, FSH, T and VEGFA levels in the indicated groups. Proestrus (P), estrus (E), metestrus (M) and diestrus (D). Data are presented as mean ± SD for three independent experiments. *P<0.05, ** P<0.01, ***P<0.001.