Research Paper
Adipocyte-derived exosomal miR-30c-5p promotes ovarian angiogenesis in polycystic ovary syndrome via the SOCS3/STAT3/VEGFA pathway
Figure 1. Characterization of adipocyte-derived exosomes. (A) Oil red O staining of differentiated 3T3-L1 cells. (B) TEM analyses of exosomes secreted by adipocytes. (C) NTA of exosomes. (D) Western blotting analyses of exosomal markers (CD9, CD63 and FABP4) in 3T3-L1 cells and adipocyte-derived exosomes. (E) Fluorescence images of HOMECs incubated with PKH26-labelled exosomes (red). The cytoskeleton was stained with phalloidin-FITC (green), and nuclei were stained with DAPI (blue).