Research Paper Volume 14, Issue 22 pp 9264—9279
CD229 interacts with RASAL3 to activate RAS/ERK pathway in multiple myeloma proliferation
Figure 3. The potential signaling pathway of CD229 for MM biology is screened by RNA-seq. (A) The volcano plot of differentially expressed genes between WT and CD229-OE MM cells. X axis, log2 fold change; Y axis, −log10 P value. (B) Pathway enrichment analysis of RNA-seq data unveiled enrichment of RAS signaling pathway. (C) RAS-GTP and total RAS expression in WT and CD229-OE MM cells were detected by RAS antibody. (D, E) WB test confirmed that p-ERK was increased in CD229-OE cells (D) and decreased in si-CD229 cells (E). The data of experiments represent Mean±SD from at least three independent experiments.