Research Paper Volume 14, Issue 24 pp 9890—9907
Sevoflurane-induced POCD-associated exosomes delivered miR-584-5p regulates the growth of human microglia HMC3 cells through targeting BDNF
Figure 4. Validation of sequencing result by real-time quantitative PCR (RT-qPCR), and BDNF was the target gene of miR-584-5p. The mRNA levels of miR-584-5p (A), miR-451a (B), miR-185-5p (C), miR-7976 (D) and miR-122-5p (E) in the non-POCD-derived exosomes and POCD-derived exosomes. N = 10. *: P < 0.05, compared with the non-POCD-derived exosomes. (F) An online tool TargetScan Human 7.1 was applied to predict that BDNF was the target gene of miR-584-5p, and dual-luciferase reporter gene assay showed that BDNF was confirmed to bind with miR-584-5p. *: P < 0.05.