Research Paper Volume 14, Issue 21 pp 8856—8875
Polydatin administration attenuates the severe sublesional bone loss in mice with chronic spinal cord injury
Figure 8. PLD inhibited the osteoclastogenic potential of bone marrow precursors isolated from chronic SCI mice. (A) The number of osteoclasts were identified by TRAP staining. (B) CTR and TRAP mRNA levels in cultured bone marrow progenitor cells. mRNA levels were determined by qRT-PCR analysis. (C) NFATc1 and c-Fos protein expression was detected by Western blotting and adjusted as relative values to GAPDH. Data are expressed as mean ± S.D.; n=4 per group; *P < 0.05, vs. the Sham group; #P < 0.05, vs. the SCI group.