Research Paper Volume 14, Issue 21 pp 8856—8875
Polydatin administration attenuates the severe sublesional bone loss in mice with chronic spinal cord injury
Figure 6. Effects of treatment with PLD on oxidative stress and vitamin D deficiency in serum and femurs of mice. MDA levels in serum (A) and femurs (B) were determined using a kit. (C) The expression level of MMP-9 in femoral tissues was detected by immunoblot. (D) Serum 25(OH)D levels were determined by radioimmune assay. (E) Femoral protein expression of VDR were determined by Western blotting and quantification analysis were shown. The protein levels of MMP-9 and VDR were adjusted as relative values to GAPDH. Data are expressed as mean ± S.D.; n=5 to 7 per group; *P < 0.05, vs. the Sham group; #P < 0.05, vs. the SCI group.