Research Paper Volume 14, Issue 21 pp 8661—8687

Figure 2. Krill oil delays senescence. (A, B) Representative images of β-gal staining in the head region in 9-day old adults, Scale bar, 20 μm. Column scatter plot representing the percentage of worms with positive senescence staining in three independent experiments (n = 50–100 individuals, column indicates mean, error bars, s.e.m, ^***p < 0.001; one-way ANOVA followed by Bonferroni’s multiple comparison test). (C, D) Images and quantification represents senescence in late passage BJ cells using β-gal staining (three independent experiments, Scale bar, 20 μm, Error bars, s.e.m; ^*p < 0.05; one-way ANOVA followed by Bonferroni’s multiple comparison test). (E) Relative p21 and TGFβ mRNA levels in BJ cells at passage 10 and 21 treated, or not, with Krill oil (100 μg/ml, 6 days) as measured by qPCR. Data represent means ± s.d., n = 3. ^*P ≤ 0.05, ^**P ≤ 0.01, ^****P ≤ 0.0001 (two-tailed Student’s t-test).