Research Paper Volume 14, Issue 21 pp 8633—8644
Epigallocatechin-3-gallate protects porcine oocytes against post-ovulatory aging through inhibition of oxidative stress
Figure 4. Epigallocatechin-3-gallate (EGCG) prevents cytochrome c release induced by aging. (A and B) Immunofluorescence images and Pearson’s correlation coefficient (r) show colocalization of cytochrome c and MitoTracker Red in the Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG oocytes. Scale bars represent 20 μm. (C) Relative mitochondrial DNA (mtDNA) copy number in the Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG oocytes. (D) Lysates from oocytes with/ without EGCG for 24 h and 48 h aging were analyzed by western blot for detection of the DRP1, LC3 and GAPDH. *p < 0.05 and **p < 0.01 indicate significant difference. Values are expressed as the mean ± SEM.