Research Paper Volume 14, Issue 21 pp 8633—8644
Epigallocatechin-3-gallate protects porcine oocytes against post-ovulatory aging through inhibition of oxidative stress
Figure 3. Epigallocatechin-3-gallate (EGCG) rescues aging-induced mitochondrial dysfunction. (A and B) Fluorescence intensity of MitoTracker Red in aging Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG oocytes. Scale bars represent 20 μM. (C) Adenosine triphosphate (ATP) levels of oocytes in the Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG groups. (D) Lysates from oocytes with/ without EGCG for 24 h and 48 h aging were analyzed by western blot for detection of the PINK1 and GAPDH. *p < 0.05 and **p < 0.01 indicate significant differences. Values are expressed as the mean ± SEM.