Research Paper Volume 14, Issue 21 pp 8633—8644
Epigallocatechin-3-gallate protects porcine oocytes against post-ovulatory aging through inhibition of oxidative stress
Figure 1. Epigallocatechin-3-gallate (EGCG) rescues aging-induced fragmentation of porcine oocytes. (A) Oocyte morphology after 48 h of aging with/without EGCG. The white dotted circle indicates fragmented oocytes. Scale bars represent 100 μm. (B) Fragmentation rate upon treatment with different concentrations of EGCG following aging for 48 h. **p < 0.01 indicates significant differences. (C) Quantitative analysis revealed chromosome misalignment after aging for 24 h and 48 h. (D) The D7 embryo morphologies in the fresh, 24 h control, and 24 h EGCG groups. (E) The blastocyst rate upon treatment with different concentrations of EGCG after aging for 24 h. Scale bars represent 100 μm. (F) Diameters of blastocyst in the fresh, 24 h control, and 24 h EGCG groups. Values are expressed as the mean ± SEM.