Research Paper Volume 14, Issue 21 pp 8763—8782

Figure 1. Hepatic HBP1 expression is low in mouse models of T2DM. (A) HBP1 protein level reduces in adult db/db mice. The protein of endogenous HBP1 was extracted from db/m and db/db mice (n=3) livers and was measured by western blotting. β-actin was detected as a loading control. Quantification was normalized to β-actin. (B) HBP1 protein level reduces in C57BL/6J mice fed by high fat diet. The protein of endogenous HBP1 was extracted from livers (n=5) of wild type (WT) C57BL/6J mice fed by normal diet (ND) and high fat diet (HFD), and was measured by western blotting. β-actin was detected as a loading control. Quantification was normalized to β-actin. (C) The HBP1 content in livers of type 2 diabetic model mice is low. Liver sections collected from db/m, db/db, ND and HFD mice (n=5) were immunohistochemically stained with anti-HBP1. Scale bar, 100 μm. Stained areas were quantitated by Image J software. Error bars represent S.D. **, p<0.01.