Research Paper Volume 14, Issue 20 pp 8498—8567

Figure 1. Flowchart of the study design and analytical steps in the present study. Gene data were retrieved from TCGA and METABRIC databases. To select targeted genes among the cadherin (CDH) family, we observed results of simultaneous higher expression levels in cancer cells than normal tissues and poorer prognoses in breast cancer patients. Afterwards, through four steps of a “differential expression analysis”, “survival analysis, protein levels, and DNA methylation”, “functional enrichment analysis”, and “tumor microenvironment”, a comprehensive analysis was conducted with the following databases and analytical methods. TCGA, The Cancer Genome Atlas; METABRIC, Molecular Taxonomy of Breast Cancer International Consortium; TIMER, Tumor IMmune Estimation Resource; CCLE, Cancer Cell Line Encyclopedia; GEPIA2, Gene Expression Profiling Interactive Analysis 2; KM, Kaplan-Meier; IHC, immunohistochemistry; BPs, biological processes; MFs, molecular functions; CCs, cellular components; KEGG, Kyoto Encyclopedia of Genes and Genomes; GSEA, Gene Set Enrichment Analysis; miRNA, micro-RNA.